Towards Modeling Liver Lobule Regeneration in 3d
نویسندگان
چکیده
Liver regeneration is a complex process, having evolved to protect animals from the consequences of liver loss caused by food toxins. We establish a computational 3D single-cell-based model of the liver lobule regenerating after intoxication by CCl4. In order to constitute a statistically representative liver lobule, we assemble information from light and confocal microscopy analyzed by an image processing chain. Furthermore we reconstruct the lobule sinusoidal blood vessel system and use direct 3D volume visualization to verify our results. This lays the foundation for understanding the complex regeneration dynamics through iterated experimentation, modeling and predictions. Preliminary simulations identified a sufficiently large cellular motility, necrotaxis as being beneficial and rapid cellular reorientation and polar cell-cell adhesion after division as essential for successful liver regeneration.
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